IAA | indole-3-acetic acid (N1) (for immunolocalization)

270 €

AS09 421 | clonality: polyclonal | host: rabbit | reactivity: Eucalyptus globulus, Pinus radiata


2 st
Item No:
AS09 421

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product information

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in  in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.


BSA-conjugated, via N1 group of indole, indole-3-acetic acid (N1)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified in PBS pH 7.4. 
Quantity 200 µg

Freeze upol arrival and store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunolocalization (IL)
Related products

AS06 193| anti- IAA

AS09 420 | anti- indole-3-acetic acid (C1') for ELISA

Secondary antibodies

Additional information

useful references for immunolocalization work:
DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121.
Shiozaki et al. (1999). Localisation of abscisic acid in grape berry by immunohitochemical techniques. J.Japan Hort.Sci.68:1-9. 
Skene et al. (1987). Model systems for the immunolocalisation of cis, trans-abscisic acid in plant tissues. Planta 172:192-199.
Sosountozov et al. (1986). Immunoelectron-microscopy localisation of abscisic acid with collodial gold on Lowicryl-embeded tissues of Chenopodium polyspermum L. Planta 168: 471-481.
Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.

application information
Recommended dilution

specific information about dilution is going to be included on the vial

Expected | apparent MW
Confirmed reactivity indole-3-acetic acid (N1) in Eucalyptus globulus, Pinus radiata
Predicted reactivity

indole-3-acetic acid (N1)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Antibody can be used for direct detection of free IAA (see reference below)

Antibody is provided in 50% glycerol. For larger quantity (1 mg) or for  ELISA kit - please inquire.

Selected references Escandón et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.
Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.
De Diego et al. (2013).Immunolocalization ofIAA andABA inroots andneedles ofradiatapine (Pinusradiata) duringdrought andrewatering. Tree Physiol., May; 33(5):537-549.

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