FBA | Fructose-bisphosphate aldolase class 2
AS13 2731 | clonality: polyclonal | host: rabbit | reactivity: Synechocystis PCC6803
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
|Confirmed reactivity||Synechocystis PCC6803|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
to be added when available, antibody released in November 2013.
From 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 (A) extracted with SDS-sample buffer and respective amounts of recombinant FBA (B) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was seconds.
Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA
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