UAGPase | UDP-GlcNAc pyrophosphorylase
AS14 2829 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, Nicotiana tabacum
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|Recommended dilution||1 : 10 000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
|Predicted reactivity||Glycne soja, Medicago truncatula, Morus notabilis, Populus trichocarpa, Theobroma cacao, Zea mays, for more species, please inquire|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
This antibody is recognizing recombinant UAGPase at 0.25 pmol.
|Selected references||Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).|
10 µg of total protein from Arabidopsis thaliana leaf (1) Hordeum vulgare leaf (2), Nicotiana tabacum (3), and recombinant UGPase 0.25 pmol (R), were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels, LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent (GE RPN 2125; Healthcare) or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 10 seconds for recombinant UAGpase and 1 minute for plant extracts.
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