UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)
AS05 086 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, C. annuum, C. sativus, F. arundinacea, H. vulgare, L. esculentum, L. chilense,Malus x domestica Borkh. c.v. Fuji, M. polymorpha, N. tabacum, O. sativa, P. glauca, Populus sp., S. tuberosum, S. sogarandinum, Triticum | cellular [compartment marker] of cytoplasm
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1: 1000 - 1: 3000 with ECL (WB), 1: 1500 (IL)
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Brassica rapa, Capsicum annuum, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji,, Marchantia polymorpha, M.vaginalis, Nicotiana tabacum, Oryza sativa, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivum|
Brassica pekinensis, Capsella rubella, Glycine max, Glycine soja, Gossipium hirsutum, Pinus taeda, Populus tremula, Ricinus communis, Saccharum officinarum, Zea mays, Vitis vinifera, Citrus sinensis, Jatropha curcas, Cucumis melo, Bambusa oldhamii, Eucalyptus grandis, Theobroma cacao, Gossypium hirsutum, Sorghum bicolor, Amorpha fruticosa, Brachypodium distachyon, for more species, please inquire
|Not reactive in||
C. merolae, diatoms
This antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana
|Selected references||Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.
Schalk et al. (2017). Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1. Proc Natl Acad Sci U S A. 2017 Mar 21. pii: 201618834. doi: 10.1073/pnas.1618834114.
Castellano et al. (2016). A pathogenic long noncoding RNA redesigns the epigenetic landscape of the infected cells by subverting host Histone Deacetylase 6 activity. New Phytol. 2016 Sep;211(4):1311-22. doi: 10.1111/nph.14001. Epub 2016 May 12.
Hsu et al. (2016). Super-resolution ribosome profiling reveals unannotated translation events in Arabidopsis. Proc Natl Acad Sci U S A. 2016 Oct 21. pii: 201614788.
Liu et al. (2016). iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. J Proteomics. 2016 Sep 2;146:80-9. doi: 10.1016/j.jprot.2016.06.031.
Bancel et al. (2015). Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. J Proteome Res. 2015 Sep 8.
Kolb et al. (2015). FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis thaliana. Plant Physiol. 2015 Feb 19. pii: pp.114.253377.
Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J. Plant Growth Regul., July 2014. (immunolocalization)
Komatsu et al. (2014). Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha L. 1. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5.
Fukayama et al. (2014). Nocturnal phosphorylation of phosphoenolpyruvate carboxylase in the leaves of hygrophytic C3 monocots. Bioscience, Biotechnology and Biochemistry.
Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7.
A 1-year-old greehouse grown plant was dissectedinto different tissues, which were then used for enzyme assays and immunoblot analyses. Equal amounts of total protein (7.5 μg) were loaded on each lane. SDS-PAGE was run on a 7.5% gel. Immunoblot was done using Amersham PVDF transfer membrane. Primary antibodies against barley UGPase were used in 1: 1000 dilution. Secondary antibodies (Amersham ECL Rabbit IgG, HRP-Linked Whole Antibody from donkey) were used at 1:10 000.
ff - female flower, mf - male flower, yl - young leaf, ml - mature leaf, sbk - stem bark, sph - stem phloem and cambium, sxy - stem xylem, rxy - root xylem
15 µg of total soluble protein extract from leaves and stems of Solanum tuberosum (1), Solanum sogarandinum (2), Lycopersicum esculentum (3), Lycopersicum chilense (4) , Arabidopsis thaliana (5) , Cucumis sativus (6) , Festuca arundinacea (7) , Nicotiana tabacum (8) and Capsicum annuum (9) were separated on 10% SDS-PAGE and blotted onto nitrocellulose . After blocking with 5% milk in TBST , blots were incubated with the primary antibody at a dilution of 1:1500 in TBST for 1h at room temperature. Following incubation and wash steps, blots were incubated withSIGMA secondary Anti-Rabbit IgG , Alkaline Phosphatase Conjugate for 1 hour at a dilution of 1:40000 . Blots were developed with the alkaline phosphatase detection system using NBT/BCIP (SIGMA).
Courtesy of Bartosz Szabala, Institute of Plant Genetics, Polish Academy of Science .
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