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Actin-11

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each
AS10 702 | Clonality: Monoclonal | Host: Mouse | Reactivity: A. thaliana, N. cataria, N. rtanjensis, N. tabacum, O. sativa, S. tuberosum, Z. mays

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AS10 702

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product information
Background

Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Immunogen

full length ACT11 (Actin-11) from Arabidopsis thaliana, accession number NP_187818.1

Host Mouse
Clonality Monoclonal
Clone IgG2b
Purity
Format Liquid, contains 50% glycerol
Quantity 50 µl
Reconstitution
Storage

Store at -20°C.
Shelf life of this product is one year from the date of shipment.

Tested applications Western blot (WB), Immunofluorescence (IF)
Related products

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AS10 681
| Anti-tubulin beta chain, rabbit antibody
AS10 680 | Anti-tubulin alpha chain, rabbit antibody
AS16 3140 | Actin-2, mouse monoclonal antibody
AS16 3141 | Actin-8, mouse monoclonal antibody
AS16 3139 | Actin-1, monoclonal antibody

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibody has been affinity purified.

application information
Recommended dilution

1 : 2000 with ECL (WB), 1: 700 (IF)

Expected | apparent MW

41.6 | 45 kDa

Confirmed reactivity

Arabidopsis thaliana, Fusarium oxysporum f.sp. cubense, Nepeta cataria, Nepeta rtanjensis, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Zea mays

Predicted reactivity

dicots including: Glycine max, Pisum sativun, Ricinus communis, monocots, trees: Picea abies, Chlamydomonas reinhardtii

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information This antibody is not suitable as a loading control for tissues from various organs of cauliflower including seeds.
Selected references Hashimoto et al. (2016). EXA1, a GYF domain protein, is responsible for loss-of-susceptibility to plantago asiatica mosaic virus in Arabidopsis thaliana. Plant J. 2016 Oct;88(1):120-131. doi: 10.1111/tpj.13265. Epub 2016 Sep 19.
Deng et al. (2015). Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Appl Microbiol Biotechnol. 2015 Sep;99(17):7189-207. doi: 10.1007/s00253-015-6768-x. Epub 2015 Jul 1.
Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.
Weiste and Dröge-Laser (2014). The Arabidopsis transcription factor bZIP11 activates auxin-mediated transcription by recruiting the histone acetylation machinery. Nat Commun. 2014 May 27;5:3883. doi: 10.1038/ncomms4883.
Weits
et al. (2014). Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway. Nat Commun. 2014 Mar 6;5:3425. doi: 10.1038/ncomms4425.


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