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FtsZ | Procaryotic cell division GTPase, cyanobacterial

350 €

AS07 217  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Cyanobacteria

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Item No:
AS07 217

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product information
Background

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.

Immunogen

Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein Q3MC27 overexpressed in E.coli.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution
For reconstitution add 200 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications
Immunofluorescence (IF), Immunolocalization (IL), Western Blot (WB)
Related products
AS09 413 | anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody
AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody
AS13 2651 | anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibody
Additional information
To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody
application information
Recommended dilution

1 : 2000 - 1: 5000 with alkaline phosphatase (WB), 1: 200 (IL) Immunogold-TEM, 1: 500 (IF)

Expected | apparent MW

44.5 | 50 kDa

Confirmed reactivity cyanobacteria including: Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Predicted reactivity

Arabidopsis thaliana, Oryza sativa, Phaeodactylum tricornutum, Prochlorococcus sp. 

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information
This antibody can be used as a loading control antibody in cyanobacteria. 

For western blot detection image and immunolocalization results - please refer to the article below.

Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).
Selected references MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571
Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.
Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)
Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.


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