FtsZ | Procaryotic cell division GTPase, cyanobacterial
AS07 217 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cyanobacteria
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1 : 2000 - 1: 5000 with alkaline phosphatase (WB), 1: 200 (IL) Immunogold-TEM, 1: 500 (IF)
|Expected | apparent MW||
44.5 | 50 kDa
|Confirmed reactivity||cyanobacteria including: Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus|
Arabidopsis thaliana, Oryza sativa, Phaeodactylum tricornutum, Prochlorococcus sp.
|Not reactive in||
No confirmed exceptions from predicted reactivity known in the moment
This antibody can be used as a loading control antibody in cyanobacteria.
For western blot detection image and immunolocalization results - please refer to the article below.
Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).
|Selected references||MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571
Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.
Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)
Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.
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