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MPK4 | Mitogen-activated protein kinase 4

379 €

AS12 2107 | clonality: polyclonal | host: chicken | reactivity: Arabidopsis thaliana

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AS12 2107

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product information
Background

MPK4 (Mitogen-activated protein kinase 4) is involved in cortical microtubules organization and stabilization, root hair development and is a negative regulator of systemic acquired resistance (SAR) and salicylic acid (SA) mediated response. Alternative name: MAP kinase 4. 

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MPK4, TAIR: AT4G01370, UniProt: Q39024

Host Chicken
Clonality Polyclonal
Clone
Purity Affinity purified IgY
Format Liquid in PBS pH 7.4 + 0.02 % sodium azide as preservative
Quantity 200 ĩg
Reconstitution
Storage

store at 4°C;  Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 2633 | anti-MPK6 | MITOGEN-ACTIVATED PROTEIN KINASE 6

Plant protein extraction buffer

Secondary antibodies

Additional information

there is no band detected by this antibody in Mpk4-2 (does not express MPK4 protein AT4G01370)  and Mpk4-2 sid 2-2 mutants. Cross-reactivity wth MPK11, 12 and 5 has been ruled out.

application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

42.9 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity dicots inlucing: Brassica napus, Glycine max, Gossypium mexicanum, Nicotiana atenuata, Petroselinum crispum, Solanum lycopersicum, Solanum tuberosum, Thelungiella halophila, monocots:
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

to be added when available, antibody released in March 2013


application example

western blot detection using anti-MPK4 antibodies


30 µg of total protein from Arabidopsis thaliana, ecotype Ws-0 extracted with extraction buffer (100mM Tricine, 3mM MgSO4, 3mM EDTA, 1mM DTT) were separated on12 % SDS-PAGE and blotted 1h to PVDF (Biorad). Blots were blocked with non-fat dry milk (Biorad) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:250 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-hen IgY(H&L), horse radish peroxidase conjugated) diluted to 1:0000 in non-fat dry milk (Biorad) for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL Plus Western Blotting Detection System (GE Healthcare) according to the manufacturers instructions. Exposure time was 30 seconds.

Courtesy of Dr. Anna Kozłowska-Makulska, Warsaw University of Life Sciences, Poland


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