Tubulin beta chain
AS10 681 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Medicago sativa
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1: 1000 (IF), 1:500 (WB)
|Expected | apparent MW||
49 | 49 kDa (Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa|
dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, microalgae:Micromonoas pusilla, Ostreococcus lucimarinus, S. cerevisiae
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added when available
|Selected references||Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113|
Tubulin beta localization in Arabidopsis thaliana epidermis cells. Tubulin beta localized to division plates visualized in green, nucleus with DAPI in blue. Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin beta priamry antibody in dilution 1: 1000 and Agrisera goat anti-rabbit IgG DyLight® 488, secondary antibody in dilution 1: 2000. Scale bar – 10 µm.
(A) The 3 days old suspension cells of Arabidopsis thaliana have been fixed for 25 minutes with 2% FA, cell wall was partaillly digested with 0.2% Dricelase in MES buffer (pH 5.1) for 30 min at 37°C and the cells were dried on coated slide to omit PM extraction. Following by incubation for 45 minutes with Agrisera anti-tubulin beta chain primary antibody in a dilution of 1: 1000, washing once and 45 min incubation with a secondary goat anti-rabbit Alexa 555 antibody, in a dilution of 1:1000 dilution (Invitrogen). Cells were counterstained witn DAPI and visialized under epi/fluiresecnet microscope (B).
Courtesy Dr. Taras Pasternak, Freiburg University, Germany
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