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Melatonin (rat)

418 €

AS09 597  |  clonality: polyclonal  |  host: rat

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AS09 597

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product information
Background

Melatonin - N-acetyl-5-methoxytryptamine, is a natural compund involved in circadian rhythms in animals and in plants. In plants it is involved in regulation of photoperiod, defence responses and acts as a scavenger of reactive oxygen species.

Immunogen

Synthetic 5-Methyohytryptamine succinic anhydride (SA) conjugated to BSA

Host Rat
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol.
Storage Store lyophilized antibodies at 4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications inDirect ELISA (ELISA) with conjugated 5-Methoxytryptamine succinic anhydride
Related products

AS09 596 | anti-melatonin rabbit antibodies

Secondary antibodies

Additional information

Specificity has been determined using ELISA competition experiments using conjugate of 5-Methoxytryptamine-Glutaric anhydride


Compounds Cross-reactivity ratio (a)
   
5-Methoxytryptamine-S-BSA 1
Free melatonin 1/ 130
5-Hydroxytryptamine-A-BSA 1/ 50 000
DL 5-Methoxytryptophan-SA-BSA 1/ >50 000
Tryptamine-SA-BSA 1/ >20 000

(a) : 5-Methoxytryptamine-GA-BSA concentration/ conjugated close structure concentration at half displacement.

application information
Recommended dilution The optimal working dilution should be determined by the investigator.
Expected | apparent MW

232.278 g/mol

Confirmed reactivity Conjugated melatonin
Predicted reactivity Conjugated melatonin
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information For example of immunocytochemistry protocol check here total IgG was purified by ammonium sulfate precipitation.
Selected references

to be added when available



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