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Melatonin

418 €

AS09 596  |  clonality: polyclonal  |  host: rabbit

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AS09 596

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product information
Background

Melatonin - N-acetyl-5-methoxytryptamine, is a natural compund involved in circadian rhythms in animals and in plants. In plants it is involved in regulation of photoperiod, defence responses and acts as a scavenger of reactive oxygen species.

Immunogen

Synthetic 5-Methyohytryptamine-glutaric anhydride-conjugated to protein carrier-BSA.

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol
Storage

store lyophilized antibodies at  4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications indirect ELISA (ELISA) with conjugated 5-Methoxytryptamine glutaric anhydride
Related products

AS09 597 | anti-melatonin rat antibodies

Secondary antibodies

Additional information

Specificity has been determined using ELISA competition experiments using conjugate of glutathione-glutaraldehyde protein.


Compounds Cross-reactivity ratio (a)
   
5-Methoxytryptamine-GA-BSA 1
5-Hydroxytryptamine-GA-BSA 1/ >7 500
DL 5-Methoxytryptophan-GA-BSA 1/ >9 500
Tryptamine-GA-BSA 1/ >50 000
5-Methoxytryptamine-G-BSA 1/ >50 000

(a) : 5-Methoxytryptamine-GA-BSA concentration/ conjugated close structure concentration at half displacement.

application information
Recommended dilution

to be determined by end user

Expected | apparent MW

232.278 g/mol

Confirmed reactivity

conjugated melatonin

Predicted reactivity

conjugated melatonin

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

for example of immunocytochemistry protocol check here
total IgG was purified by ammonium sulfate precipitation

Selected references

to be added when available



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