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PhyA | Phytochrome A

345 €

AS07 220   |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum

PRODUCT INFORMATION IN PDF

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Item No:
AS07 220

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product information
Background

Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.

Immunogen

KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis  thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 198 | anti-FT/TSF | flowering locus T and twin sister of FT
AS16 3158 | anti-PhyB | phytochrome B protein
AS16 3147 | anti-SPA1 | supressor of PhyA-105
AS12 2112 | anti-PIF5 | phytochrome interacting factor 5

Additional information

in vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in this paper.

application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

124 kDa

Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum
Predicted reactivity

Brassica rapa, Lathyrus sativus, Fragaria ananassa, Glycine max, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

careful sample collection is adviced to assure the best results with this antibody

Selected references Pierto-Dapena et al. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. J Exp Bot. 2017 Feb 16. doi: 10.1093/jxb/erx020.
Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.

application example

10 µg of total protein extracted with PEB (AS08 300) from etiolated seedlings of Arabidopsis thaliana (1) was separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 2h (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PhyA (AS07 220, 1:1000, 19.5h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, standard sensitivity).

Application example


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