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AKINB3 | SNF1-related protein kinase regulatory subunit beta-3

195 €

AS15 2859 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana

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AS15 2859

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product information
Background AKIN ß3 (SNF1-related protein kinase regulatory subunit beta-3) is a regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta 3 sequence, UniProt: Q9ZUU8, TAIR: AT2G28060
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)
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AS09 463 | Anti-AKIN beta gamma, rabbit antibody
AS10 919 | Anti-AKIN10  SNF1-related protein kinase catalytic subunit alpha KIN10, rabbit antibody
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AS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibody

collection of antibodies to proteins involved in signal transduction
Additional information
application information
Recommended dilution 1: 1000 (WB)
Expected | apparent MW

12.7 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brasica rapa, Capsella rubella, Ricinus communis, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known
Additional information
Selected references To be added when available, antibody released in April 2016. 

Application example


western blot using anti-AKIN beta 3 polyclonal antibodies
Recombinant AKIN beta 3 were separated by 10% SDS-PAGE and transferred to a PVDF membrane using semi-dry blotting (for 1h). Blots were blocked with 5% low-fat milk powder in TBS-T (0.01% Tween 20) for 1h at room temperature (RT) with agitation. The blot was then incubated overnight at 4 °C in the primary antibody at a dilution of 1: 1 000 in 1% low-fat milk powder in TBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at RT with agitation. The blot was then incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in 1% low-fat milk powder in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min and developed for 2 min with Pico reagent (Pierce) according to the manufacturer's instructions. Exposure time to film was 10 min.

Courtesy of PhD student Sander Hulsmans, KU Leuven, Belgium

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