AKING1 | SNF1-related protein kinase regulatory subunit gamma 1
AS09 613 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 2000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana|
|Not reactive in||
Chlamydomonas reinhardtii, Vitis vinifera
|Selected references||Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
Ramon et al. (2013). The hybrid Four-CBS-Domain KINβγ-subunit functions as the canonical γsubunit of the plant energy sensor SnRK1. Plant j. April 1.
10 ug of Arabidopsis thaliana Columbia WT and KIN gamma KO seedlings were grinded in MOPS loading buffer and heated for 5 min at 95°C. Samples were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly
twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody, diluted to 1:4 000 in for 1h at RT with agitation.
The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 15 seconds.
Courtesy of Dr. Matthew Ramon
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