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RACK1A | Receptor for activated C kinase 1A

265 €
Buy 2 items of this product for 198 €/each
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AS11 1810  | clonality: polyclonal  |  host: rabbit  |  reactivity:Arabidopsis thaliana, Thellungiella salsuginea

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Item No:
AS11 1810

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product information
Background

RACK1A is a major component of RACK1 regulatory proteins playing a major role in multiple signal transduction pathways. Synonymes: Receptor for activated C kinase 1A, WD-40 repeat auxin-dependent protein ARCA.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RACK1A protein sequence O24456 At1g18080

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

other antibodies to signal transduction pathway components

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 with standard ECL (WB)

Expected | apparent MW

35 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Thellungiella salsuginea
Not reactive in

Nicotiana benthamiana, Zea mays

Additional information

To be added when available

Selected references Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.

application example

western blot using anti-RACK1 antibody

20 µg of total protein from Arabidopsis thaliana total cell extract was separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation.Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.


Courtesy of Dr. Sascha Laubinger, ZMBP, Germany


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