RACK1A | Receptor for activated C kinase 1A
AS11 1810 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Thellungiella salsuginea
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|Recommended dilution||1 : 2000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Thellungiella salsuginea
|Not reactive in||
Nicotiana benthamiana, Zea mays
|Selected references||Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.
20 µg of total protein from Arabidopsis thaliana total cell extract was separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation.Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.
Courtesy of Dr. Sascha Laubinger, ZMBP, Germany
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