P-Tyr | Phosphotyrosine
AS10 705 | clonality: monoclonal | host: mouse | reactivity: phosphotyrosine
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW|
|Confirmed reactivity||Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.
|Selected references||Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):
Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):
Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
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