P-Tyr | Phosphotyrosine
AS10 705 | clonality: monoclonal | host: mouse | reactivity: phosphotyrosine
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW|
antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.
antibody is not species specific
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.
(WB): Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.
(IP): Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.
(IF): Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
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