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P-Tyr | Phosphotyrosine

383 €

AS10 705   | clonality: monoclonal  |  host: mouse  |  reactivity: phosphotyrosine

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AS10 705

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product information
Background

Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.

Immunogen

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide

Host Mouse
Clonality Monoclonal
Clone IgG1 (clone G104)
Purity
Format Liquid
Quantity 100 ĩg
Reconstitution
Storage

store at -20°C for one year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC)
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Secondary antibodies

Additional information

protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW
Confirmed reactivity antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.
Predicted reactivity

antibody is not species specific

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.

Selected references Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):
Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):
Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.


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