SUMO1 | Small ubiquitin-like modifier protein 1
AS08 308 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 1000 - 1: 5000 for standard ECL (WB)
|Expected | apparent MW||
10.97 | 12 kDa
|Confirmed reactivity||Arabidopsis thaliana|
dicots including: Glycine max, Solanum lycopersicum, Nicotiana tabacum, Pisum sativum, monocots including: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Antibodies will also detect SUMO2 protein.
|Selected references||Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.
Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
Liu et al. (2014). SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis. BMC Plant Biol. 2014 Jun 3;14:153. doi: 10.1186/1471-2229-14-153.
Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.
Arabidopsis thaliana total cell extract HA-proSUMO1 (HA-epitope and pro-Small Ubiquitin like MOdifier protein1 (1), empty vector only (2), were separated on 15% gel, SDS -PAGE and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 1 000 anti-AtSUMO1 antibody (AS08 308) for 1 h, followed by incubation with 1: 15 000 secondary anti-rabbit antibodies (1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
Note: signal in the empty vector lane comes from endogenous SUMO1 detected by the antibody.
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