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TPS1 | Trehalose-6-phosphate synthase 1

265 €
Buy 2 items of this product for 198 €/each
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AS12 2635 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS12 2635

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product information
Background

TPS1 (Trehalose-6-phosphate synthase 1), EC=2.4.1.15 is an enzyme required for normal embryo development, vegetative growth and transition to flowering. It is involved in the regulation of glucose sensing. Expressed in seedlings, leaves, roots, stems, flowers and siliques. Up-regulated during seed development. Alternative names: alpha-trehalose-phosphate synthase [UDP-forming] 1.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPS1, UniProt: Q9SYM4, TAIR: AT1G78580

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS05 086 | UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker), rabbit antibody
collection of antibodies involved in carbohydrate metabolism

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 5000 with standard ECL (WB)

Expected | apparent MW

105.9 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Camelia sinensis, Vitis vinifera
Not reactive in

monocots

Additional information

so far this antibody was not used on endogenous extracts from Arabidopsis thaliana

Selected references

to be added when available, antibody released in March 2014.


Application example

western blot using anti-TPS1 antibodies

Total protein from Arabidopsis thaliana wilde type (1,2), knowck out mutant (3) and overexpression line (4) were extracted with 50 mM HEPES/NaOH (pH 7.5), 1 mM EDTA, 1 mM DTT (+Protease Inhibitor cocktail) and denatured at 100°C for 5 min. Proteins were separated on 10 % SDS-PAGE and blotted overnight at 4°C to PVDF using wet tank transfer. Blots were blocked with 5 % low fat milk in 1xTBS-T for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 2 % low fat milk in 1xTBS for 1h at RT with agitation. The blot was washed as above and developed. Exposure time was 10 seconds.

Courtesy of Dr. Vasiliki Zacharaki, Umeå Plant Science Centre, Sweden


western blot using anti-TPS1 antibodies

10 µg of total protein from Nicotiana benthamiana leaves expressing AtTPS1-GFP were separated on 10% SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Crude: non-purified material. Purified: immunoprecipitated with anti-GFP beads. Blots were blocked with 5% skimmed milk powder dissolved in TBS-T (0.1 % Tween 20)  at 4°C ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at room temperature (RT) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Immobilon Western Chemiluminescent HRP reagent  according to the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy of Dr. Takeo Sato, Hokkaido University, Japan


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com