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PLD | phospholipase D, Biotin conjugated

350 €

AS09 557 | clonality: polyclonal | host: rabbit | reactivity: Brassica oleracea

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Item No:
AS09 557

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product information
Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 ml
Reconstitution For reconstitution please add 1 ml of sterile destilled water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Related products

AS09 556 | anti-PLD phospholipase D

AS09 558 | anti-PLD phospholipase D (affinity purified)

Plant protein extraction buffer

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
Recommended dilution

1: 1000 - 1:  20 000 for techniques listed above

Expected | apparent MW

92 kDa

Confirmed reactivity

Brassica oleracea

Predicted reactivity

dicots including: Arabidopsis thaliana, Cucumis melo, Ricinus communis, trees: Populus balsamifera

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Selected references

to be added when available



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