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PLD | Phospholipase D

218 €

AS09 556 | clonality: polyclonal | host: rabbit | reactivity: Brassica oleracea

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Item No:
AS09 556

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product information
Background

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.

Immunogen

phospholipase D isolated and purified from Brassica oleracea

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Related products

AS09 557 | anti-PLD phospholipase D (biotinylated), rabbit anitbody

AS09 558 | anti-PLD phospholipase D (affinity purified), rabbit antibody

AS12 2364 | anti-PLDA1/2 | phospholipase D alpha 1/2, rabbit antibody with broader species reactivity

Plant protein extraction buffer

Secondary antibodies

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
application information
Recommended dilution 1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

92 kDa

Confirmed reactivity Brassica oleracea
Predicted reactivity Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Selected references

to be added when available



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