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RPS14 | Ribosomal protein S14 (mitochondrial)

265 €
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AS16 4094 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS16 4094

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product information
Background RPS14 | Ribosomal protein S14 (mitochondrial) is a structural constituent of ribosome and is localized in mitochondria.
Immunogen KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana RPS14 protein sequence. UniProt: O64695   TAIR:
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Tested applications Western blot (WB)
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antibodies to proteins involved in translation
Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

15.6 | 16 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Amborella trichopoda, Anthurium amnicola, Brassica juncea, Brassica oleracea var. botrytis, Capsella rubella, Carica papaya, Castilleja paramensis, Cycas taitungensis, Eruca sativa, Erythranthe guttata, Eschscholzia californica, Flagellaria indica, Glycine max, Gossypium raimondii, Guzmania lingulata, Heuchera parviflora var. saurensis, Hesperelaea palmeri, Liriodendron tulipifera, Luzula sylvatica, Medicago truncatula, Nelumbo nucifera, Nicotiana tabacum, Nymphaea tetragona, Oenothera berteroana, Pisum sativum, Phaseolus angularis , Phoenix dactylifera, Poa saltuensis subsp. languida, Populus canescens, Raphanus sativus, Silene latifolia, Sinapis arvensis, Typha latifolia, Vicia faba, Vigna radiata, Vitis vinifera
Not reactive in
Additional information
Selected references To be added when available, antibody released in February 2017.

Application example

Western blot using anti-S14 antibodies

30 µg of mitochondrial fraction from Arabidopsis thaliana  extracted with buffer (sucrose, tetrasodiumpyrophosphate, EDTA, KH2O4, PVP-40, BSA, pH 7.5) and denatured with 56°C for 15 min at 650 rpm were separated on 12 % SDS-PAGE and blotted overnight (12V) to PVDF using wet transfer. Blots were blocked with 5% skimmed powder milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the purified anti S14 serum at a dilution of 1: 1 000 for 1,5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 2 min with WesternBright Quantum HRP substrate (Advansta). Exposure time was ~ 60 seconds.

Courtesy of Dr. Małgorzata Kwaśniak-Owczarek, University of Wrocław, Poland

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