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H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28)

454 €
AS16 3184 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, rat, Xenopus sp.

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AS16 3184

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product information
Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, trimethylation of K27 is associated with gene silencing, whereas pS28 is associated with mitotis and immediate early genes.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), Dot blot (Dot), immunofluorescence (IF), western blot (WB)
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collection of antibodies to epigenetics
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0.01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP), 1: 500 - 1:1000 (Dot), 1: 200 (IF), 1: 50 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity human
Predicted reactivity C.elegans, chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

ChIP using anti-H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28)  polyclonal antibodies

Chromatin Immunoprecipitation: using anti-H3K27me3S28p antibodies. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 μg of H3K27me3S28p and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.

dot blot using anti-H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28)  antibodies'

Dot Blot: using anti-H3K27me3S28p antibodies. Lane 1: S28p/K27 unmodified. Lane 2: S28p N-Term. Lane 3: S28p C-term. Lane 4: K27Me3. Lane 5: S28p/K27Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody at 1 μg/ml for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.


immunofluorescence using anti-H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28) polyclonal antibodies

Immunofluorescence: using anti-H3K27me3S28p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:200 dilution for 1 h at RT. Secondary antibody: Dylight ®488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K27me3S28p is nuclear and chromosomal. Staining: Histone H3K27me3S28p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight®550 (red).

western blot using anti-H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28)  polyclonal antibodies

Western Blot: using anti-H3K27me3S28p antibodies. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo cell lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

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