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H3R2me1 | Histone H3 (monomethylated Arg2)

454 €
AS16 3167 clonality: polyclonal  |  host: rabbit  |  reactivity: C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.

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AS16 3167

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product information
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Immunogen KLH-conjugated synthetic peptide which surrounds the R2me1
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS16 3197 | anti-anti-H3core, monoclonal antibodies
AS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodies
AS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodies
AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710

collection of antibodies to epigenetics
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

C.elegans, human

Predicted reactivity D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

western blot using anti-H3R2me1 polyclonal antibodies
Western blot of H3R2me1 antibody. 30 μg of HeLa histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-H3R2me1 polyclonal antibodies on C.elegans embryo lysate


Western blot of H3R2me1 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.