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H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)

454 €
AS16 3185 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, rat, Xenopus sp.

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AS16 3185

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product information
Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. The dimethylation of both arginine 2 (H3R2me2) and lysine 4 (H3K4me2) of H3 are both known marks to have opposing affects. R2me2 maintains transcriptional silence by silencing Set1 mediated K4 methylation, in which K4 methylation is normally associated with active chromatin. The protein arginine methyltransferase PRMT6 can methylate H3R2 in vivo, and overexpression of this enzyme downregulates Hox and Myc dependent genes, both of which are targets of H3K4 methylation.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), Dot blot (Dot), immunofluorescence (IF), western blot (WB)
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collection of antibodies to epigenetics
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0.01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP), 1:1000 (Dot), 1: 200 (IF), 1: 50 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

human

Predicted reactivity C.elegans, chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application information

ChIP using anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4) polyclonal antibodies

Chromatin Immunoprecipitation: using anti-H3R2me2sK4me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R2me2sK4me2 and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.

dot blot using anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4) polyclonal antibodies
Dot Blot: using anti-H3R2me2sK4me2 antibodies. Lane 1: R2me2. Lane 2: R2me2/K4me2. Lane 3: K4me2. Load: 0.1, 1, 0.5, 5, and 10 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

immunofluorescence using anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)  polyclonal antibodies

Immunofluorescence: using anti-H3R2me2sK4me2 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3R2me2sK4me2 is nuclear and chromosomal. Staining: Histone H3R2me2sK4me2 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).

western blot using anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4) polyclonal antibodies
Western Blot: using anti-H3R2me2sK4me2 antibodies. 30 μg of C. elegans embryo cell lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4) polyclonal antibodies
Western Blot: using anti-H3R2me2sK4me2 antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

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