H3R8me2(asym) | Histone H3 (asym-dimethylated Arg8)
AS16 3177 | clonality: polyclonal | host: rabbit | reactivity: chicken, C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.
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|Recommended dilution||chromatin immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)|
|Expected | apparent MW||
|Confirmed reactivity||C.elegans, human|
|Predicted reactivity||chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.|
|Not reactive in||No confirmed exceptions from predicted reactivity known at the moment|
|Additional information||To be added when available|
|Selected references||To be added when available, antibody released in February 2016.|
Chromatin Immunoprecipitation using anti-H3R8me2(asym) antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(asym) and 20ul of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR, and normalized to the input chromatin.
Immunofluorescence using anti-H3R8me2(asym) antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: H3R8me2(asym) is nuclear and chromosomal. Staining: H3R8me2(asym) is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).
Western Blot using antiH3R8me2(asym) antibodies. Lane 1: HeLa Histone extracts. 2. NIH-3T3 extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
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