H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8)
AS16 3178 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, Plant, Rat, Xenopus sp.
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|Recommended dilution||2-5 ĩg/million cells (ChIP), 2 ĩg/ml (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Caenorhabditis elegans, Human|
|Predicted reactivity||Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||To be added when available, antibody released in February 2016.|
Chromatin Immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(sym) to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input.
Western Blot using anti-H3R8me2(sym) antibodies. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Western Blot using anti-H3R8me2(sym) antibodies. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 μg per lane. Primary antibody incubated at 2 μg/mL overnight at 4°C. Secondary antibody IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
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