H3S10pT11p | Histone H3 (p Ser10, p Thr11)
AS16 3179 | clonality: polyclonal | host: rabbit | reactivity: chicken, C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.
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|Recommended dilution||2-5 ĩg/million cells (ChIP), 1 : 200 (IF), 1 : 200 (IHC), 1 : 500 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Caenorhabditis elegans, Human|
|Predicted reactivity||Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||To be added when available, antibody released in February 2016.|
Immunofluorescence using anti-H3S10pT11p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: H3S10pT11p antibody at a 1:200 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: H3S10pT11p is nuclear and chromosomal. Staining: H3S10pT11p is expressed in green, nuclei are counterstained with Dapi (blue).
Western Blot using anti-H3S10pT11p antibodies. 30 μg C. elegans embryo lysate. Primary antibody: H3S10pT11p at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Western Blot using anti-H3S10pT11p antibodies. 30 μg HeLa histone extracts. Primary antibody: H3S10pT11p at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
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