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H3S10pT11p | Histone H3 (p Ser10, p Thr11)

454 €
AS16 3179 | clonality: polyclonal | host: rabbit | reactivity: chicken, C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.

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AS16 3179

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product information
Background When confronted with DNA damage or the lack of certain kinases like Chk1 and Aurora1, histone H3 is phosphorylated at T11, causing a reduction in acetylation of the same histone at K9 and ultimately leading to a reduction of active transcription in affected cells. When the T11 phosphorylation is combined with a typical S10 phosphorylation that is critical in chromosome condensation in metaphase, the effect on transcriptional regulation is amplified further. Phosphorylation of H3S10 is decreased by T11 phosphorylation. Thus, detection of the combined modification is critical in determining transcriptional effects, especially when contrasted against single modifications of H3.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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collection of antibodies to epigenetics
Additional information
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP), 1: 200 (IF), 1: 200 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

C.elegans, human

Predicted reactivity chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

immunofluorescence using anti-H3S10pT11p | Histone H3 (p Ser10, p Thr11)  polyclonal antibodiesi
Immunofluorescence using anti-H3S10pT11p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: H3S10pT11p antibody at a 1:200 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: H3S10pT11p is nuclear and chromosomal. Staining: H3S10pT11p is expressed in green, nuclei are counterstained with Dapi (blue).

western blot using anti-H3S10pT11p | Histone H3 (p Ser10, p Thr11)  polyclonal antibodies

Western Blot using anti-H3S10pT11p antibodies. 30 μg C. elegans embryo lysate. Primary antibody: H3S10pT11p at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.

western blot using anti-H3S10pT11p | Histone H3 (p Ser10, p Thr11)  polyclonal antibodies
Western Blot using anti-H3S10pT11p antibodies. 30 μg HeLa histone extracts. Primary antibody: H3S10pT11p at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.