H3T3pK4ac | Histone H3 (ac Lys4, p Thr3)
AS16 3170 | clonality: polyclonal | host: rabbit | reactivity: C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.
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|Recommended dilution||2-5 µg/million cells (ChIP), 1: 1000 (Dot), 1: 100 (IF), 1: 50 (IHC), 1: 500 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||C.elegans, human|
|Predicted reactivity||D. melanogaster, mouse, plant, rat, Xenopus sp.|
|Not reactive in||No confirmed exceptions from predicted reactivity known at the moment|
|Additional information||To be added when available|
|Selected references||To be added when available, antibody released in February 2016.|
Dot Blot using anti-H3T3pK4ac antibodies. Lane 1: T3p. Lane 2: T3pK4me1. Lane 3: T3pK4me2. Lane 4: T3pK4ac. Lane 5: T3pR2me2s. Lane 6: K4 unmodified. Lane 7: K4me1. Lane 8: K4me2. Lane 9: K4me3. Lane 10: K4ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.
Immunofluorescence using anti-H3T3pK4ac antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4ac is nuclear and chromosomal. Staining: H3T3pK4ac is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight® 550 (red).
Western Blot using anti-phospho-acetyl-Histone H3 antibodies (H3T3pK4ac). Lane 1: HeLa histone extracts. Lane 2. NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 00 for 45 min at RT.
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