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H3T6p | Histone H3 (p Thr6)

464 €
AS16 3173 | clonality: polyclonal | host: rabbit | reactivity: C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.

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AS16 3173

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product information
Background Phosphorylation as a post-translational modification on histone H3 modulates the ability of proteins to recognize and bind to the H3 tail. When additional modifications to H3 are present, in addition to the native T6 phosphorylation, there is an amplified effect.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), Dot blot (Dot), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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collection of antibodies to epigenetics
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP), 1: 1000 (Dot), 1: 100 (IF), 1: 200 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

C.elegans, human

Predicted reactivity D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

Dot blot using anti-H3T6p antibodies
Dot blot using anti-H3T6p antibodies. Lane 1: H3T6un. Lane 2: H3T6p. Load: 1, 0.3, 0.1, 0.03 picomoles of peptide. Primary antibody used at a 1:1000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

IF using anti-H3T6p antibodies
Immunofluorescence using anti-H3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T6p is nuclear and chromosomal. Staining: H3T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

western blot using anti-H3T6p antibodies


Western Blot using anti-H3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-H3T6p antibodies on HeLa extracts.


Western Blot using anti-H3T6p antibodies on 30 μg HeLa histone extracts. Primary antibody: incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.