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H3T6pK9me2 | Histone H3 (dimethylated Lys9, p Thr6)

454 €
AS16 3175 | clonality: polyclonal | host: rabbit | reactivity: chicken, C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.

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AS16 3175

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product information
Background H3K9me2 is a histone post-translational modification that is enriched in transcriptionally inactive regions. It is also important in cellular differentiation and maturation. Phosphorylation of histone H3 is also associated with mitosis and meiosis, and seems to be related to developmental changes.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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collection of antibodies to epigenetics
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP),  1: 100 (IF), 1: 200 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

human

Predicted reactivity C.elegans, chicken, D. melanogaster, human, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

ChIP using anti-H3T6pK9me2 | Histone H3 (dimethyl Lys9, p Thr6)  polyclonal antibodies

Chromatin Immunoprecipitation using anti-H3T6pK9me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me2 antibody to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.

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