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m6A | N6-methyladenosine (polyclonal)

265 €
AS17 4121 clonality: polyclonal |  host: rabbit  |  reactivity: human, mouse, other species

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AS17 4121

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product information
Background m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.
Immunogen LPH (Limulus polyphemus hemocyanin)- conjugated N6-methyladenosine (m6A)
Host Rabbit
Clonality Polyclonal
Clone
Purity
Format
Quantity 50 µg
Reconstitution
Storage Store at -20°C; for long term storage -80°C is recommened; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot)
Related products AS17 4120 | Anti-m6A | N6-methyladenosine (monoclonal) antibodies

collection of antibodies to epigenetics
Additional information This antibody is purified on Protein A and provided in PBS pH 7.4 with 0.05 % sodium azide and 0.05 % ProClin300.
application information
Recommended dilution 1: 400 (Dot)
Expected | apparent MW
Confirmed reactivity human, mouse and other species
Predicted reactivity Broad species range.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information
Selected references To be added when available, antibody released in March 2017.

Application example

 
Dot blot using anti-m6A polyclonal antibodies


 
Dot blot: using anti-m6A rabbit polyclonal antibody with synthetic peptides containing different mofified bases in a range from 50 to 150 pmol) which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A polyclonal antibody (dilution 1:400) in TBS-T with a 1 % non-fat-dry milk.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com