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m6A | N6-methyladenosine (monoclonal)

361 €
AS17 4120 clonality: monoclonal  |  host: mouse  |  reactivity: human, mouse, other species

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AS17 4120

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product information
Background m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.
Immunogen BSA-conjugated N6-methyladenosine (m6A)
Host Mouse
Clonality Monoclonal
Clone class IgG1
Purity Protein A purified
Format Liquid
Quantity 50 µg
Reconstitution
Storage Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot), RNA Immunoprecipitation (RIP) (RIP)
Related products AS17 4121 | Anti-m6A | N6-methyladenosine (polyclonal) antibodies

collection of antibodies to epigenetics
Additional information This antibody is purified on Protein A and provided in PBS pH 7.4 with 0.05 % sodium azide.
application information
Recommended dilution 1 : 500 (Dot), 1-10 ĩg (RIP)
Expected | apparent MW
Confirmed reactivity Human, Mouse and other species
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references To be added when available, antibody released in March 2017.

Application example

 
Dot blot using anti-m6A antibodies



 
Dot blot: using anti-m6A mouse monoclonal antibody with DNA oligonucleotide containing different m6A modified bases (in a range from 4 to 100 pmol) and a negative control which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A monoclonal antibody (dilution 1:500) in TBS-T with a 10 % non-fat-dry milk.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com