DCL3 | Dicer-like protein 3
AS12 2103 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Arabidopsis thaliana|
|Not reactive in||
Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)
to be added when available, antibody released in August 2012.
50 µg of total protein from Arabidopsis thaliana total cell extract which came from the ground, frozen powder and has been directly transferred to 2x Laemmli sample buffer was separated on 15 % SDS-PAGE and blotted over night at 64 mA to nitrocellulose (wet transfer). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 (1) and 1: 500 (2) 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 min.
Courtesy of Dr. Sascha Laubinger, ZMBP, Germany
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