RpoB | RNA polymerase beta subunit (chloroplast) (maize)

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AS15 2867A | Clonality: polyclonal | Host: rabbit | Reactiviy: Zea mays


24 st
Item No:
AS15 2867A

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product information
Background RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates. 
Immunogen His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 100 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS15 2866 | anti-RpoA | RNA polymerase alpha subunit (chloroplast), rabbit antibodies
AS15 2867 | anti-RpoB | RNA polymerase beta subunit (chloroplast), rabbit antibodies

AS15 2868 | anti-RpoC1 | RNA polymerase beta' subunit (chloroplast) , rabbit antibodies
collection of antibodies to proteins involved in transcription

Additional information
application information
Recommended dilution 1 : 500 (WB)
Expected | apparent MW

121 kDa

Confirmed reactivity Zea mays
Predicted reactivity Alloteropsis semialata, Coleataenia prionitis, Digitaria exilis, Echinochloa crus-galli var. crus-galli , Eragrostis tef, Microlaena stipoides, Miscanthus sacchariflorus, Oryza sativa, Phragmites australis, Potamophila parviflora, Rhynchoryza subulata, Saccharum officinarum, Setaria italica, Sorghum bicolor, Stipa lipskyi, Sporobolus michauxianus, Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody does not work on total cell extracts. Stromal fraction has to be used.

This antibody is detecting recombinant RpoB.
Selected references To be added when available, antibody released in May 2016.

Application example

western blot using anti-RpoB

Stroma, thylakoid, nuclei and total protein from Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with ECL advance. Exposure time was 120 seconds.

Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA

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