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COR14b | Cor14b-encoded cold regulated protein

345 €

AS06 169  |  clonality: polyclonal  |  host: rabbit  |  reactivity: monocots including Hordeum vulgare

PRODUCT INFORMATION IN PDF

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Item No:
AS06 169

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product information
Background

Cor14b is a nuclear encoded protein localized in chloroplast whose expression level has been associated with frost and photoinhibition tolerance in monocots.

Immunogen

KLH-conjugated synthetic peptide conserved in cor14b sequence of Hordeum vulgare, cor14c UniProt Q8H0Q4 and Triticum aestivum, REP13 of Secale cereale 

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Additional information
application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

16 kDa

Confirmed reactivity Hordeum vulgare
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Rapacz et al. (2008). The effect of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high light treatment in cold. Ann. Bot. 101: 689-699.

application example

 

Total cell extract of (1-3) Hordeum vulgare  after 1 day of cold acclimation (4-6) Hordeum vulgare  after 6 hours of cold acclimation, (7-9) Hordeum vulgare  non-acclimated plants were separated on  SDS-PAGE. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation.  Blots were developed using ECL.

 

western blot using anti-cor14b antibodies
 

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