ZCP2 | Zinc Chaperone Protein
AS12 1848 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii | zinc deficiency marker
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
70 | 100 kDa (probably due to glycosylation)
|Confirmed reactivity||Chlamydomonas reinhardtii|
|Predicted reactivity||Emiliania huxleyi|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.
|Selected references||Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.|
Chlamydomonas reinhardtii soluble proteins (10 µg) were separated on a 7.5% SDS-PAGE gel and blotted to nitrocellulose for 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was
decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in PBS-T + 1% milk at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech ) diluted to 1:3000 in PBS-T + 1% milk for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T + 1% milk at RT with agitation, then rinsed with TBS (pH 7.5), and developed.
Courtesy Dr. Dudley Page, UCLA, USA
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