ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

Cu/ZnSOD | Cu/Zn superoxide dismutase

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS10 652  |  clonality: polyclonal  |  host: hen  |  reactivity: L. longiflorum, O. europaea, O. sativa, S. lycopersicum, S. oleracea

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
17 st
Delivery:
Shipping:
Item No:
AS10 652

Info: Product suggestions Read reviews
product information
Background

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Immunogen

15 amino acids synthetic peptide designed from olive pollen cytosolic Cu/Zn cDNA consensus sequences (GenBank Accessions EU250757 to EU250797), UniProt Q8L5E0

Host Chicken
Clonality Polyclonal
Clone
Purity Total IgY
Format Liquid in 10mM Tris, 150 mM NaCl, 0.02% sodium azide
Quantity 100 ĩl
Reconstitution
Storage

store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase

AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase

AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD

AS09 524 | anti-MnSOD | manganese superoxide dismutase

collection of antibodies to plant stress proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Total IgY concentration is 2.3 mg/ml.

Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.

application information
Recommended dilution

1 : 1500 with Alexa 488 (WB)

Expected | apparent MW

15.3 | 16 kDa (Olea europaea L.)

Confirmed reactivity Arabidopsis thaliana (leaves), Lilium longiflorum, Olea europaea (pollen), Oryza sativa, Spinacia oleracea (leaves), Solanum lycopersicum, Zea mays
Predicted reactivity

Ananas ananas, Betula pendula, Camellia sinensis, Codonis lanceolata, Cucurbita ficifolia, Helianthus sp., Hordeum vulgare, Lycopersicum esculentum, Plantago major, Populus trichocarpa, Solanum nigrum, Solanum tuberosum, Solidago sp., Vitis vinifera

Not reactive in

Nicotiana tabacum

Additional information

Note: Antibody recognizes two to three isoforms of Cu/Zn SOD in olive pollen depending on the olive cultivar.

Selected references Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.

application example

25 µg of total protein from Olea europaea pollen were separated on 12% acrylamide gels and blotted onto PVDF. Membranes were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Cu/Zn SOD (AS10 652, 1:1500, overnight, 4º C) and secondary antibody anti-chicken IgG conjugated to Alexa 488 (1:2000, 1 h) (Molecular Probes, Invitrogen). Signal was detected with a PharosSX fluorescence scanner (Bio-Rad).


The antibody recognizes a protein of c.a. 16 kDa. and another band likely corresponding to the protein dimer.

Courtesy Dr. Juan de Dios Alche, CSIC, Spain

 

  western blot using hen anti-Cu Zn SOD antibody

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com