MnSOD | Manganese superoxide dismutase
AS09 524 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. maritima, B.napus, N. cataria, N. rtanjensis, O. sativa, P. sativum, S. tuberosum
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|Recommended dilution||1 : 2000-1 : 5000 (WB)|
|Expected | apparent MW||
25 | 25 kDa
|Confirmed reactivity||Arabidopsis thaliana, Armeria maritima, Brassica napus, Iris pumila, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Pisum sativum, Solanum tuberosum|
|Predicted reactivity||Gossipium mexicanum, Hordeum vulgare, Musa acuminata, Picea sitcHensis, Populus balsamifera sub. trichocarpa, Raphanus sativus, Triticum aestivum, Vitis vinifera, Zea mays|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19
Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.
Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.
Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.
Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
5 µg (1,2), 10 µg (3, 4) of total protein from Pisum sativum were separated on 12% SDS-PAGE and blotted 30 min. to PVDF. Blots were blocked (in 5% fat free milk) immediately following transfer in for 1h at RT with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2 000 overnight in 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 5 min in TBS-T at RT with agitation. Blots were incubated in secondary antibody (anti- IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 for 1h at RT with agitation. The blots were washed 4 times for 5 min in TBS-T and 2 times for 5 min in TBS and developed for 1 min with ECL detection reagent according to the manufacturers instructions. Exposure time was 60 seconds.
Courtesy Dr. Elżbieta Romanowska, Warsaw University, Poland
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