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AO | ascorbate oxidase

418 €

AS09 384  | clonality:polyclonal | host:rabbit | reactivity: Cucurbita sp.

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AS09 384

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product information
Background

Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA).  Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Immunogen

ascorbate oxidase purified from Cucurbita sp.

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.2
Quantity 1 mg
Reconstitution For reconstitution add 0.5 ml of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Tested applications Western blot (WB), ELISA (ELISA), immunohistochemistry (IHC)
Related products

AS06 184 | anti-ascorbate oxidase rabbit antibody

AS09 383 | anti-ascrobate oxidase biotinylated rabbit antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography. 

application information
Recommended dilution

1: 1000 (WB), 1: 1000 - 1: 30 000 (ELISA)

Expected | apparent MW

61 | 45 kDa (Cucurbita pepo)

Confirmed reactivity Cucurbita sp.
Predicted reactivity

dicots including Glycine max, monocots including Oryza sativa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available



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