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AO | Apoplastic ascorbate oxidase

345 €

AS06 184  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Cucurbita sp., N. tabacum, S. tuberosum, Z. mays  |  cellular [compartment marker] of chloroplast

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Item No:
AS06 184

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product information
Background

Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA).  Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Immunogen

ascorbate oxidase purified from Cucurbita sp.

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS09 383 | anti-ascorbate oxidase biotinylated antibody

Additional information

IgG concentration is 4 µg/µl

application information
Recommended dilution

1:2 000 with standard ECL (WB)

Expected | apparent MW

61 | 45 kDa (Cucurbita pepo)

Confirmed reactivity

Cucurbita sp., Nicotiana tabacum, Solanum tuberosum, Zea mays

Predicted reactivity

dicots including Glycine max, Cucumis melo, monocots including Oryza sativa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

antibody can be used as a marker of apoplast

Selected references

Kielbowicz et al. (2008) The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J. Expo Bot. 59:2191-2203.



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