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Gamma-ECS | Gamma glutamylcysteine synthase

345 €

AS06 186  | clonality: polyclonal  |  host: rabbit  |  reactivity: N.tabacum, S. lycopersicum, Z. mays

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AS06 186

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product information
Background

Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays  gamma-ECS Q8W4W3

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunolocalization (IL)
Related products

collection of antibodies to plant stress proteins

Additional information

total IgG concentration is 5.2 µg/ µl

application information
Recommended dilution

1: 5000 with standard ECL (WB), 1: 5000  (IL)

Expected | apparent MW

50 kDa

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabcum, Solanum lycopersicum, Zea mays

Predicted reactivity

dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

antibodies has been used in immunolocalization on Arabidopsis thaliana

Selected references Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.



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