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GSH | Glutathione (rat)

418 €

AS09 595  |  clonality: polyclonal  |  host: rat

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AS09 595

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product information
Background

Glutathione (GSH) is a nucleophilic tripeptide which helps to protect cells from reactive oxigen species such as free radicals and peroxides.

Immunogen

Synthetic glutathione  conjugated to BSA protein carrier.

Host Rat
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol
Storage

store lyophilized antibodies at  4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), immunohistochemistry (IHC)
Related products

AS09 594 | anti-GSH | glutathione rabbit antibodies

Secondary antibodies

Additional information

Specificity has been determined using ELISA competition experiments using conjugate of glutathione-glutaraldehyde protein.

Compounds Cross-reactivity ratio (a)
   
Glutathione-G-BSA 1
Cysteine-G-BSA 1/ >10 000
Glutamate-G-BSA 1/ >10 000
Glycine-G-BSA 1/ >10 000

(a) : Glutathione-G-BSA concentration /unconjugated or conjugated analogs concentration at half displacement.

G = Glutaraldehyde, BSA = Bovine Serum Albumin

application information
Recommended dilution

1: 2000 - 1: 5000 (ELISA), 1: 500 - 1: 1 000 (IHC)

Expected | apparent MW
Confirmed reactivity glutathione
Predicted reactivity

glutathione

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

for example of immunocytochemistry protocol check here
total IgG was purified using ammonium sulfate precipitation

Selected references Amara et al. (1994). Antibodies to reduced glutathione.Brain Res. 659:237-242.


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