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HRP | Horseradish peroxidase

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS16 3951  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. rusticana, A. thaliana, C. elegans 

PRODUCT INFORMATION IN PDF

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AS16 3951

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product information
Background

Horseradish peroxidase (HRP) is a secretory plant peroxidase that catalyzes the oxidation of small aromatic substrates, such as plant hormone and lignin precursors by hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.

Immunogen

Native peroxidase isolated and purified from horseradish.

Host

Rabbit

Clonality

Polyclonal

Clone
Purity Serum
Format

Lyophilized

Quantity
Reconstitution

For reconstitution please add 50 μl of sterile destilled water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

ELISA (ELISA), Western Blot (WB)

Related products

AS09 549 | anti-HRP | Horseradish peroxidase (affinity purified), rabbit antibody

Antibodies against Tag proteins

Additional information
application information
Recommended dilution

1: 1000 - 1: 5000 (WB)

Expected | apparent MW

38.6 kDa

Confirmed reactivity

Armoracia rusticana, Arabidopsis thaliana, Caenorhabditis elegans 

Predicted reactivity

Armoracia rusticana

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references

To be added when available, antibody released in December 2016.


Application example
Western blot using anti-HRP antibody

Proteins were isolated from poplar T89, barley, Arabidopsis thaliana, horseradish root mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 45 µg of total proteins and 45-7.5 µg of HRP were loaded into each lane and separated on 12% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody AS09 602, followed by washing steps as above. Membrane was developed for 2 min with GE Healthcare Western Blotting Detection Reagent according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 100 s.

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