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3-nitroY | Nitrotyrosine (25 ĩl)

194 €

AS10 706-25  | clonality: monoclonal  |  host: mouse  |  reactivity:nitrotyrosine

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AS10 706-25

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product information
Background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species.

Immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
acetamido) propionic acid

Host Mouse
Clonality Monoclonal
Clone IgG2A
Purity Protein G purified
Format Liquid
Quantity 25 ĩl
Reconstitution
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Related products

AS10 706-100 | anti-nitrotyrosine mouse antibody, 100 µg

Secondary antibodies

Additional information

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4,  0.09 % sodium azide, 50 % glycerol

application information
Recommended dilution The optimal working dilution should be determined by the investigator.
Expected | apparent MW
Confirmed reactivity Dog, Human, Mouse, Rat
Predicted reactivity Higher plants
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL.

Selected references Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.


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