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ACD | alpha crystalline domain of sHSP

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS15 3029  |  Clonality: polyclonal  |  Host: rabbit  |  Reactivity: recombinant ACD domain od sHSP

PRODUCT INFORMATION IN PDF

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AS15 3029

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product information
Background ACD (alpha crystalline domain)
Immunogen KLH-conjugated peptide derived from sequences of ACD domain
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution

For reconstitution add 50 ĩl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Additional information
application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

16.6 kDa

Confirmed reactivity

recombinant sHSP with ACD domain

Predicted reactivity ACD domain of sHSP
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references To be added when available, antibody released in May 2016. 

application example

 

 

 Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis  PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody  (1: 10 000 from Abcam).

 

 Western blot using anti-Hsp16.6 antibodies


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