ATPase AAA2 domain
AS11 1754 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Synechococcus sp. PCC7942
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|Recommended dilution||1 : 2000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Synechococcus sp. PCC 7942|
|Predicted reactivity||AAA2 domain containing proteins including Saccharomyces cerevisiae HSP104.|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
to be added when available, antibody released in October 2011.
0.3 µg of total protein from Synechococcus sp. PCC7942 (1) and 20 µg of leaf soluble proteins from 3-week old Arabidopsis thaliana leaves (2) were separated on 3-8 % Tris-acetate NuPage gel (invitrogen) and blotted 1,5 h to supported introcellulose. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in 1xTBS-T for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:75 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.
Courtesy of Dr. A. Clarke, Göteborg University, Sweden
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