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CDJ1 | chloroplast DnaJ homolog 1

345 €

AS06 179  |  clonality: polyclonal  | host: rabbit  |  reactivity:Chlamydomonas reinhardtii

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AS06 179

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Background

CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

Immunogen

fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB) immunoprecipitation (IP)
Related products

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

collection of antibodies to chloroplast proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1. 3000 with standard ECL (WB)

Expected | apparent MW

40 | 40 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Ostreococcus sp.

Not reactive in

higher plants

Additional information

western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl

Selected references Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.


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