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HSF1 | Heat shock factor 1

345 €

AS06 178  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomons reinhardtii

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Item No:
AS06 178

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product information
Background

HSF1 is a key regulator of the stress response. It becomes hyperphosphorylated following heat shock (results in the shifting of the HSF1 protein to slightly higher MW) and HSF1 protein rapidly accumulates during heat stress. HSF1 appears to be constitutively present as high molecular weight complexes, presumably trimers. It also interacts with cytosolic HSP70A.

Immunogen

C-terminal part of Chlamydomonas reinhardtii HSF1 (Exons 7 to 9), UniProt: A1YTN3

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1. 3000 with standard ECL (WB)

Expected | apparent MW

78.4 | 100 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

higher plants

Additional information

unspecific cross-reactions at 40-50 kDa

Selected references

Schulz-Raffelt et al. (2007).. Heat shock factor 1 is a key regulator of the stress response in Chlamydomonas. The Plant Journal  52: 286-295



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