HSP17.6 | Cytosolic class I heat shock protein 17.6 (rabbit antibody)
AS07 254 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. tequilana, C. sativus, I. pumilla (perennial monocot), P. ternata, P. sylvestris, S. vulgaris
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Agave tequilana var. Weber, Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris|
|Predicted reactivity||Higher plants, Nicotiana tabacum|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Additional information||There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.|
|Selected references||Murano et al. (2017). A purine-type heat shock protein 90 inhibitor promotes the heat shock response in Arabidopsis. Plant Biotechnology Reports April 2017, Volume 11, Issue 2, pp 107–113.
Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]
Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression.
Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)
Florentin et al. (2013). Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev. Dyn. Oct; 242(10):1121-33.
15 µg of total protein from (HS) heat shocked Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.6 were separated on 15%SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).
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