HSP17,6 | Cytosolic class I heat shock protein 17,6 (rabbit antibody)

Product no: AS07 254

AS07 254 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequilana, B. juncea, Citrus sp., C. sativus, I. pumilla (perennial monocot), P. ternata,  P. sylvestris, S. vulgaris, V. faba

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  • Product Info
  • Immunogen:

    Recombinant Arabidopsis thaliana Hsp17.6 CI (class one) P13853, At1g53540

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 17.6 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Agave tequilana var. Weber, Brassica juncea,  Citrus sp., Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris, Solanum tuberosum, Vicia faba
    Predicted reactivity: Higher plants, Fraxinus sp., Nicotiana tabacum
    Species of your interest not listed? Contact us
    Not reactive in: Polyscias elegans
  • Application Examples
  • Application example

    western blot using anti-Hsp17.6 antibodies

    15 µg of total protein from (HS) heat shocked Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.6 were separated on 15%SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).

  • Additional Information
  • Additional information: This product can be sold containing ProClin if requested
    Additional information (application): There are six total class I genes, Essentially this antibody might react to some extent with all of them, But does not react with class II, organelle, or any other shsp classes
  • Background
  • Background:

    Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

  • Product Citations
  • Selected references: Swetha et al. (2021) Single and Combined Salinity and Heat Stresses Impact Yield and Dead Pericarp Priming Activity. Plants (Basel). 2021 Aug 8;10(8):1627. doi: 10.3390/plants10081627. PMID: 34451672; PMCID: PMC8399105.
    Siddiqui et al. (2020). Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress. Journal of Hazardous MaterialsVolume 398, 5 November 2020, 122882
    McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263
    Kato et al. (2019). Induction of the heat shock response in Arabidopsis by chlorinated 1,4-naphthoquinones. Plant Growth Regul (2019). https://doi.org/10.1007/s10725-019-00477-3.
    Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
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    Number of reviews: (1)
    Damián Balfagón | 2020-07-20
    0 | 0
    I used it in Citrus and worked very well.

Accessories

AS07 255 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequiliana var. Weber, C. sativus, S. vulgaris, P. ternata

319 €
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Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

218 €
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