HSP17.6 | Cytosolic class I heat shock protein 17.6
AS07 254 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. tequilana, C. sativus, I. pumilla (perennial monocot), P. ternata, P. sylvestris, S. vulgaris
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1: 1000 (WB)
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Agave tequilana var. Weber, Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris|
|Predicted reactivity||higher plants, Nicotiana tabacum|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
|Additional information||There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.|
|Selected references||Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]
Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression.
Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)
Florentin et al. (2013). Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev. Dyn. Oct; 242(10):1121-33.
15 µg of total protein from (HS) heat shocked Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.6 were separated on 15%SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).
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