HSP17.7 | Cytosolic class II heat shock protein 17.7
AS07 255 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. tequiliana var. Weber, C.sativus, S. vulgaris, P. ternata
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Agave tequiliana var. Weber, Cucumis sativus, Medicago sativa, Pinellia ternata, Silene vulgaris
|Not reactive in||
|Selected references||Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.
15 µg of total protein from (HS) heat shocked (38ºC/2h) Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.7 were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000). Development was done ECL (Thermo Scientific) according to the recommendations of the manufacturers.
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