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HSP17.7 | Cytosolic class II heat shock protein 17.7

265 €
Buy 2 items of this product for 198 €/each
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AS07 255 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. tequiliana var. Weber, C.sativus, S. vulgaris, P. ternata

PRODUCT INFORMATION IN PDF

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Item No:
AS07 255

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product information
Background

Hsp17.7 belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Immunogen

full length recombinant protein produced in E. coli and purified by conventional methods (no affinity tag). Arabidopsis thaliana Hsp17.7 CII (class two) O65376, At1g12030

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibody

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibody

collection of antibodies to plant heat shock proteins

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

17.7 kDa

Confirmed reactivity Arabidopsis thaliana, Agave tequiliana var. Weber, Cucumis sativus, Medicago sativa, Pinellia ternata, Silene vulgaris
Predicted reactivity

dicots

Not reactive in

Oryza sativa

Additional information
Selected references Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.

application example

 

15 µg of total protein from (HS) heat shocked (38ºC/2h) Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.7 were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam). Development was done ECL (Thermo Scientific) according to the recommendations of the manufacturers.
Image was acquired with a 10 sec exposure time on X-ray film (Kodak). Band at ~38 kDa represents and SDS resistant dimer.


western blot using anti-HSP17.7 antibodies

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