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HSP21 | Chloroplastic heat shock protein

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS08 285 |clonality: polyclonal | host: rabbit | reactivity: A.thaliana, N. tabacum

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AS08 285

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product information
Background

HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

Immunogen

Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21 P31170, At4g27670

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibody

AS08 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibody

AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7

collection of antibodies to plant heat shock proteins

Additional information

Recommended load per well 20 µg of total protein

This product can be sold containing proClin if requested.

application information
Recommended dilution

1: 3000 with standard ECL (WB)

Expected | apparent MW

25 | 21 kDa

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabacum

Predicted reactivity

Arabidopsis thaliana

Not reactive in

Chlamydomonas reinhardtii

Additional information

Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.

Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

Selected references Lämke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.
Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]
Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.
Hai-Dong Yu et al. (2012). Downregulation of Chloroplast RPS1 Negatively Modulates Nuclear Heat-Responsive Expression of HsfA2 and Its Target Genes in Arabidopsis. Plos Genetics.


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